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RNA stores tau reversibly in complex coacervates

Fig 2

Enrichment of tRNA in PhotoActivatable Ribonucleoside-enhanced Individual-nucleotide resolution UV Cross-Linking ImmunoPrecipitation and high-throughput sequencing (PAR-iCLIP) data.

(A) Cross-Linking ImmunoPrecipitation (CLIP) cDNA reads from tau expressed in human-induced pluripotent stem cell (hiPSC)-derived neurons (neuron CLIP) and from tau expressed in human embryonic kidney (HEK) cells (HEK cell CLIP) that aligned to the chr15.tRNA4-ArgTCG tRNA were found in all CLIP samples, and demonstrated a similar pattern of crosslinking. (B) Analysis of cross-linked sites along tRNA secondary structure demonstrates the anticodon preference, in which the anticodon (colored red) region is designated as position 1–3 for alignment purpose. The colored illustration of tRNA secondary structure is displayed as an inset, and below the x axis in one dimension. The numerical data used in (B) are included in S1 Data.

Fig 2

doi: https://doi.org/10.1371/journal.pbio.2002183.g002