Water diffusion closely reveals neural activity status in rat brain loci affected by anesthesia
Fig 6
Effects of H-80 infusion in the Central Medial thalamus nucleus (CM) on the Apparent Diffusion Coefficient (ADC), Local Field Potentials (LFPs) and awake response threshold.
(A) Percentage of animals exhibiting an awake response during CM electrical stimulation after the CM infusion with −80 milliosmole (mOsm) hypotonic artificial cerebrospinal fluid (aCSF) (H-80; n = 6). (B) Average ADC change in the CM and cingulate cortex (Cg) before and after H-80 or aCSF infusion in the CM (n = 6 for each condition). Average time course of ADC change at the CM (C) and Cg (D) with the infusion of aCSF and H-80. (E) Representative local field potentials (LFP) signals at CM and Cg during CM infusion with aCSF (upper) or H-80 (low). (F) Total LFP power (frequency range: 1–70 Hz) in CM and Cg before and after CM infusion with H-80 (n = 6) or aCSF (n = 6). Time course and bar plots exhibit mean ± the standard error of the mean (SEM). ** p < 0.01 (Paired t-test between pre and post), # p < 0.05, ## p < 0.01 (Fisher’s exact test). Data for minimum amplitudes of individual rats can be found in S5 Data. ADC data of individual rats found in S3 Data. LFP power data can be found in S4 Data.