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Commensal bacteria and essential amino acids control food choice behavior and reproduction

Fig 2

Flies specifically increase yeast appetite upon single essential amino acid (eAA) deprivation.

(A) Feeding preference of flies kept on holidic medium or holidic medium lacking all amino acids (AAs), all nonessential amino acids (neAAs), or single eAAs in the context of no neAAs. Circles represent yeast preference in single assays, with a line representing the median and whiskers representing the interquartile range. n = 26. Significance was tested using the Kruskal–Wallis test followed by Dunn’s multiple comparison test. (B) Cumulative intake measurement of yeast extract and sucrose using the capillary feeder (CAFE) assay. Flies were prefed a holidic diet containing either all AAs, no AAs, all AAs except valine (Val), or all AAs except arginine (Arg). Dots represent means and error bars represent the standard error of the mean. n = 10. Significance was tested using the unpaired t test with Bonferroni correction for the intake volume at 4 h. For yeast extract intake in (B), Val and Arg deprivation have the same effect when compared to the complete holidic medium. There was also no significant effect of the different diets on sucrose intake. Not significant (ns) p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Underlying data used in this Fig are provided in S1 Data.

Fig 2

doi: https://doi.org/10.1371/journal.pbio.2000862.g002