Commensal bacteria and essential amino acids control food choice behavior and reproduction
(A) The holidic diet allows the analysis of the impact of specific nutrients contained in yeast. (B) Yeast preference of flies kept on yeast-based medium and medium without yeast (sucrose medium). (C) Yeast preference of flies kept on holidic medium and holidic medium lacking different specific nutrients. (D) Amino acid (AA) preference of flies kept on full holidic medium and holidic medium lacking all AAs. (E and F) Yeast (E) and AA (F) preference of flies kept on complete holidic medium or holidic medium lacking all AAs, all nonessential amino acids (neAAs), or all eAAs. In (B), (C), and (E), flies were given the choice between sucrose and yeast. In (D) and (F), flies were either given the choice between holidic medium lacking AAs (sucrose option) or the holidic medium lacking sucrose (AAs option) and in (D) the sucrose option and holidic medium without sucrose and AAs (–AAs in choice). Circles represent yeast or AA preference in single assays, with a line representing the median and whiskers representing the interquartile range. n = 12–18. Significance was tested using the Kruskal–Wallis test followed by Dunn’s multiple comparison test. (B–F) Not significant (ns) p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. In this and the following Figs, green signifies diets with full eAA content and blue signifies diets lacking one or more eAAs. Underlying data used in this Fig are provided in S1 Data.