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Stochastic assembly produces heterogeneous communities in the Caenorhabditis elegans intestine

Fig 2

Rare migrants grow and colonize the C. elegans intestine.

(A) External bacterial density during feeding controls feeding rate (bacteria eaten/worm/h, grey points and line), which in turn reflects the colonization rate (average number of internal bacteria acquired/worm/h, black points and line). Worms were colonized in a well-mixed liquid culture with E. coli MC4100 E3350 or E3322 as a food source; no differences were observed in colonization ability between strains at 1 h, and data were pooled. Average internal CFU/worm measurements were obtained from batch digests of 100–200 worms after 1 h of colonization. (B) In the absence of new colonization (see Methods), growth of unlabeled E. coli MC4100 in the C. elegans intestine is described by a logistic model (black line, average CFU/worm; red line, fit of the logistic equation with r ≈ 1.5 d-1 and K ≈ 2 x 105 CFU/worm). Open squares indicate number of individual uncolonized worms digested each day (CFU/worm = 0). In both panels, bars indicate mean ± SD estimated by assuming Poisson error of colony counts. See S1 Data.

Fig 2