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Quantifying the Determinants of Evolutionary Dynamics Leading to Drug Resistance

Fig 2

The drug-specific dose-sensitivity is robust to genetic perturbations and correlates with fitness variability.

(A) Dose-response curves for eight antibiotics; circles (●) show trimethoprim; pluses (+), tetracycline; downward triangles (▼), chloramphenicol; stars (★), nitrofurantoin; squares (■), ciprofloxacin; leftward triangles (◄), cefoxitin; triangles (▲), mecillinam; rightward triangles (►), ampicillin. Dose-sensitivity n is shown (Materials and Methods). (B) Scatterplot of dose-sensitivity n and DFE width (IQR); Pearson’s ρ = 0.96, p = 1.3 × 10−4; n error bars show standard deviation of replicates; DFE width error bars show bootstrap 95% confidence interval (Materials and Methods). Horizontal dashed line shows DFE width in the absence of drug (cf. Fig 1B). Gray line shows a linear relation as a guide to the eye. (C) Mecillinam dose-response curves for 78 arbitrary deletion mutants (purple; see S1 Data) and 17 WT replicates (black). (D) Same data as in C with concentration rescaled to IC50 and growth rate response rescaled to g0 (Materials and Methods). See also S1 Fig Numerical data is in S1 Data.

Fig 2

doi: https://doi.org/10.1371/journal.pbio.1002299.g002