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Quantifying the Determinants of Evolutionary Dynamics Leading to Drug Resistance

Fig 1

Fitness variability changes drastically in the presence of different antibiotics.

(A) Sample growth curves (optical density over time) of wild type (WT; black) and gene deletion mutants pdxJ (green) and iscS (red); yellow lines are exponential fits (Materials and Methods). (B) Histogram of growth rates (i.e., approximated DFE) of ~4,000 gene deletion strains in the absence of drug; histogram of 476 WT replicates is outlined in black. (C) DFE in the presence of the antibiotics trimethoprim, nitrofurantoin, tetracycline, chloramphenicol, ciprofloxacin, mecillinam, cefoxitin, and ampicillin (Table 1); vertical black lines show median of WT replicates; drugs were used at concentrations inhibiting WT growth by one-third. Growth rates are normalized to median of WT in the absence of a drug. The interquartile ranges (IQRs) of the DFEs are shown in Fig 2B. Numerical data is in S1 Data.

Fig 1

doi: https://doi.org/10.1371/journal.pbio.1002299.g001