Population Diversification in a Yeast Metabolic Program Promotes Anticipation of Environmental Shifts
Figure 2
Glucose is consumed before galactose despite the presence of a subpopulation of cells with an active GAL pathway.
Wild-type cells were exposed to 0.1% glucose and 0.1% galactose simultaneously at time zero. (A) Representative dynamic measurements of glucose, galactose, and the fraction of ON cells (FON) for wild-type expressing pGAL10-Venus. Highlighted box indicates plateaued region. The lines through the glucose and galactose data represent fitted Hill functions. (B) FON as a function of time as scored by pGAL1, pGAL2, pGAL7, and pGAL10 promoter fusions to Venus. (C) Plot of FON scored by either pGAL1, pGAL2, pGAL7, or pGAL10 versus glucose concentration. (D) Plot of FON scored by either pGAL1, pGAL2, pGAL7, or pGAL10 versus galactose concentration. Data can found in S1 Data.