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Inhibitor of the Tyrosine Phosphatase STEP Reverses Cognitive Deficits in a Mouse Model of Alzheimer's Disease

Figure 4

TC-2153 does not induce neuronal cell death.

Cortical cells were incubated with TC-2153 (1, 10, and 100 µM) for 1 h along with positive controls: glutamate (100 µM), SDS (0.02%), and Triton-X-100 (0.15%) (A), and at multiple time points (1 h, 3 h, 24 h, and 48 h) (B). Bovine LDH was used as a LDH-positive control. The media was collected and analyzed for LDH. The assay quantitatively measures LDH, a stable cytosolic enzyme that is released upon cell lysis. Released LDH in culture supernatants is measured with a 30-min coupled enzymatic assay, which results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells.

Figure 4