Hypoxia-Inducible Factor-2α Is an Essential Catabolic Regulator of Inflammatory Rheumatoid Arthritis
Figure 5
Normal immune system development and effector function of CD4+ T cells in Epas1+/− mice.
(A) Representative flow cytometric analysis of CD4+ and CD8+ T-cell populations in the lymph nodes of WT and Epas1+/− DBA/1J mice. (B) Populations of TH1, TH2, and TH17 cells differentiated from uncommitted CD4+ T cells of WT and Epas1+/− DBA/1J mice. (C) IL17A-producing cells identified by flow cytometry (left), and levels of secreted IL17A determined by ELISA (right), from lymphocytes (LN) and splenocytes (SP) of WT and Epas1+/− DBA/1J mice (n = 8 mice per group) under CIA conditions. (D) mRNA levels of the indicated cytokines in total knee synovial cells isolated from Epas1+/− DBA/1J mice under CIA conditions or in Ad-Epas1–injected mice (n = 10). The NI condition and Ad-C injection were used as controls. Values are means ± SEM (*p<0.01, **p<0.005, ***p<0.0005).