Electron Tomography and Simulation of Baculovirus Actin Comet Tails Support a Tethered Filament Model of Pathogen Propulsion
Figure 1
Live cell imaging of baculovirus actin comet tails.
(A) Overview of GFP-actin expressing B16 melanoma cell that was infected with baculoviruses tagged with mCherry. (B) Relative distribution of actin and associated proteins along baculovirus comet tails. Tail length averaged 3.8±1.8 µm (s.d., n = 111 individual tails) and was normalized to 1.0. Curves were fitted using a centered sixth order polynomial function. Shown are 95% confidence bands for each dataset. For details, see Figure S1. Bar, 10 µm.