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Different Levels of Catabolite Repression Optimize Growth in Stable and Variable Environments

Figure 4

Fitness tradeoffs between rapid adaptation and MaxRs.

Many evolved strains show reduced lag phases in variable environments (LG; LG + maltose or LG + galactose). (A) Growth pattern of the ancestral strain in either stable glucose conditions (HG or in media that require a shift from glucose to a less preferred carbon source (LG; LG + maltose or LG + galactose). (B) Similar analysis as panel (A) of Isolate 1 from Figure 3A. Note that the reduction in growth speed (lag phase) is much less pronounced than for the ancestral strain shown in panel (A). (C) Dividing the growth rates of the evolved clone by the ancestral reveals that the evolved clone grows more slowly than the ancestral strain, except during the lag phase, where the evolved isolate shows a much higher growth rate. This shorter lag phase is responsible for the increased GMR relative to the ancestral strain. (D) The MaxR and the GMRs are anticorrelated. Each point represents the geometric mean of the GMRs in all different conditions used in this study (LG, LG + galactose, LG + maltose, HG) versus the geometric mean of all MaxRs in the same conditions. Error bars represent standard deviations. The grey square represents the ancestral strain.

Figure 4