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The Interaction between a Sexually Transferred Steroid Hormone and a Female Protein Regulates Oogenesis in the Malaria Mosquito Anopheles gambiae

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MISO knockdown decreases egg production to virgin levels.

(A) Mated females injected with dsRNA were blood fed and allowed to lay eggs 3 d post-blood-feeding for 4 nights. Control females (dsLacZ) laid on average 82.5 eggs, while dsMISO oviposited a statistically significant lower number of eggs (65.4). The data are representative of three independent replicates. (B) Virgin or mated females injected with dsRNA were blood fed, and eggs developed inside the ovaries were counted 3 d post-blood-feeding without allowing oviposition. Mated dsLacZ produced on average 77.8 eggs, while virgin dsLacZ and mated dsMISO produced a statistically significant lower number of eggs (62.3 and 60.4, respectively). The data are representative of six independent replicates.

Figure 1

doi: https://doi.org/10.1371/journal.pbio.1001695.g001