Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis
(A) Flow chart demonstrating the steps used to purify RBP63 (DLA2). (B) SDS-PAGE and Coomassie Blue staining of proteins at various stages of purification (upper panel) and UV cross-linking (UV-CL) of RBP63 to radiolabeled psbA 5′ UTR RNA (bottom panel). HepFT/HepW, flow through/wash fractions from heparin Sepharose; HepE 150, 500, and 1000, eluates obtained with 150, 500, and 1,000 mM KCl from heparin Sepharose, respectively; pA FT/W, flow-through/wash fraction from poly(A)-Sepharose column; pA E150, 550, and 1000, eluates obtained with 150, 500, and 1,000 mM KCl, respectively. The RBP63 protein that eluted with 500 mM KCl from poly(A)-Sepharose is marked by an arrow.