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The Cell Cycle Timing of Centromeric Chromatin Assembly in Drosophila Meiosis Is Distinct from Mitosis Yet Requires CAL1 and CENP-C

Figure 3

CID assembly in female meiosis I.

(A) Changes in the amount of CID at centromeres during meiosis I in Drosophila females. Ovaries were fixed and stained with anti-CID antibody (green), anti-C(3)G antibody to mark the synaptonemal complex (red), and DNA is stained with DAPI (blue). Oocyte nuclei are circled in white. Staging is as described in [41],[63]. Scale bar: 5 ┬ÁM. (B) Quantification of total centromeric CID fluorescence intensity per oocyte nucleus during prophase of meiosis I in Drosophila females. Bars, standard errors. N = 273 total cells. N = 15, cystoblast; 26, region 2b; 40, stage 1; 36, stage 2; 53, stage 3; 54, stage 4/5; 33, stage 6/7; 16, stage 8/9.

Figure 3