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Bet Hedging in Yeast by Heterogeneous, Age-Correlated Expression of a Stress Protectant

Figure 5

Old cells are Tsl1-GFP abundant.

(A) TSL1-GFP yeast stained with the bud scar stain WGA-TRITC are passed though a cell sorter to monitor co-fluorescence. Shown is the WGA-TRITC fluorescence of cells binned by Tsl1-GFP fluorescence. p-Values are a comparison to all cells, Wilcoxon-Mann-Whitney test: ***, p<1×10−10. (B) Sorted WGA-TRITC stained TSL1-GFP cells are counted for bud scars. Shown is the cumulative percentage of all cells (grey) and cells in the top 1% Tsl1-GFP fluorescence bin (green). The 1% Tsl1-GFP cells have significantly more bud scars, Wilcoxon-Mann-Whitney test, p<1×10−7. (C) Population demography accounts for some expression “noise.” Shown is the protein-expression noise (DM in yeast permissive dextrose media from [4]) of genes binned by logarithm of their age expression ratio, the average expression in young cells over the average expression in old cells [73]. Error bars indicate SEM; p-values are a comparison to all colonies; Wilcoxon-Mann-Whitney test: *, p<0.05; **, p<0.001.

Figure 5