Advertisement

< Back to Article

Facilitation of AMPA Receptor Synaptic Delivery as a Molecular Mechanism for Cognitive Enhancement

Figure 4

Enhanced postsynaptic excitatory transmission in neurons treated with FGL.

(A) Average AMPA/NMDA ratios for treated and untreated cells. AMPAR-mediated responses were recorded at −60 mV, and NMDAR-mediated responses were recorded at +40 mV. The p value was determined using the Mann-Whitney test. (B) Average AMPA/GABA ratios for treated and untreated cells. AMPAR-mediated responses were recorded at −60 mV, and GABA-mediated responses were recorded at +0 mV. NMDAR were blocked with DL-AP5. The p value was determined using a t test. Representative traces appear above the corresponding bars. N, number of cells. (C) Average NMDA/GABA ratios for treated and untreated cells. NMDAR-mediated responses were recorded at −60 mV in the absence of Mg2+ and in the presence of CNQX to block AMPARs. GABA-mediated responses were recorded at 0 mV. The p value was determined using a t test. Representative traces appear above the corresponding bars. N, number of cells. (D) Paired-pulse facilitation (PPF) in FGL and control neurons. The values denote the ratio of the second EPSC amplitude to the first EPSC amplitude. PPF was tested for 50-, 100-, 200-, and 400-ms interstimulus intervals. Insets. Sample trace of evoked AMPAR-mediated synaptic responses with an interstimulus interval of 50 ms. N, number of cells. Scale bars: 10 pA, 50 ms.

Figure 4

doi: https://doi.org/10.1371/journal.pbio.1001262.g004