A Yeast Model of FUS/TLS-Dependent Cytotoxicity
Figure 4
Deletion of arginine methyl transferase does not rescue FUS/TLS toxicity nor change its localization.
(A) Empty vector and N-terminus GFP-tagged FUS/TLS on pYES2CT were transformed into yeast arginine methyl transferase deletion strain rmt1Δ, rmt2Δ, and its isogenic wild type BY4743 (WT). Spotting assay was performed to observe toxicity from the above yeast strains. (B) Expression of the proteins from above strains was induced by 2% galactose for 6 h. Localization and aggregation of the protein was visualized by fluorescence microscopy.