A User's Guide to the Encyclopedia of DNA Elements (ENCODE)
The image shows selected ENCODE and other gene and transcript annotations in the region of the human TP53 gene (region chr17:7,560,001–7,610,000 from the Human February 2009 (GRCh37/hg19) genome assembly). The annotated isoforms of TP53 RNAs listed from the ENCODE Gene Annotations (GENCODE) are shown in the top tracks of the figure, along with annotation of the neighboring WRAP53 gene. In black are two mRNA transcripts (U58658/AK097045) from GenBank. The bottom two tracks show the structure of the TP53 region transcripts detected in nuclear polyadenylated poly A+ RNAs isolated from GM12878 and K562 cells. The RNA is characterized by RNA-seq and the RNAs detected are displayed according to the strand of origin (i.e. + and −). Signals are scaled and are present at each of the detected p53 exons. Signals are also evident at the U58658  and AK097045  regions located in the first 10 kb intron of the p53 gene (D17S2179E). The U58658/AK097045 transcripts are reported to be induced during differentiation of myeloid leukemia cells but are seen in both GM12878 and K562 cell lines. Finally the p53 isoform observed in K562 cells has a longer 3′UTR region than the isoform seen in the GM12878 cell line.