The Perilipin Homologue, Lipid Storage Droplet 2, Regulates Sleep Homeostasis and Prevents Learning Impairments Following Sleep Loss
Figure 5
Lsd2 mutant flies mimic the phenotypes seen in starved cyc01 flies.
(A) A representative section of Lsd2rev head stained with Oil Red O. Head fat bodies are denoted by arrowheads. (B) A representative section of Lsd251 head stained with Oil Red O. (C) Lsd251 mutants (n = 22) do not exhibit a homeostatic response following 12 h of sleep deprivation while their genetic background control, Lsd2rev (n = 41), do exhibit a sleep rebound following sleep loss. *p = 0.012; Student's t test. (D) Amylase mRNA levels of Lsd251 mutants are significantly lower than genetic background controls, Lsdrev, following 12 h of sleep deprivation. Data are presented as percentage from untreated siblings (2 replicates of n = 20 heads/group). *p<.05, Student's t test. (E) Learning is not impaired in Lsd251 mutants (n = 7) following 12 h of sleep deprivation compared to untreated controls (n = 8); p = 0.37 Student's t test. (F) Learning is impaired in Lsd2rev flies (n = 15) following 12 h of sleep deprivation compared to untreated controls (n = 15).* p = 0.036 Student's t test.