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IL-6 and IL-10 Anti-Inflammatory Activity Links Exercise to Hypothalamic Insulin and Leptin Sensitivity through IKKβ and ER Stress Inhibition

Figure 6

Role of hypothalamic IL-10 in the control of energy intake during obesity.

Western blots showing hypothalamic lysates from Wistar rats; (A) IL-1ra and (B) IL-10 expression in the hypothalamus. (C) IL-10 mRNA in the hypothalamus was examined using real time PCR assay. (D) 12 h food intake (kcal) in obese rats under resting conditions after intrahypothalamic infusion of different doses of recombinant IL-10. Western blots showing hypothalamic lysates from Wistar rats; (E) IL-10 expression after ASO IL-10 treatment in obese animals. (F) Intrahypothalamic treatment with ASO IL-10 blocked the anorexigenic response mediated by (F) insulin and (G) leptin in exercised obese animals or obese animals at rest injected with recombinant IL-6. Western blots showing hypothalamic lysates from Wistar rats; (H) IKKβ, (I) PERK, and (J) IRS-1Ser307 phosphorylation after ASO IL-10 treatment or after acute recombinant IL-10 infusion. (K) Insulin-induced Akt serine phosphorylation and (L) leptin-induced STAT3 tyrosine phosphorylation in the hypothalamus after ASO IL-10 treatment or after acute recombinant IL-10 infusion. (M) Basal levels of Akt serine phosphorylation and (N) STAT3 tyrosine phosphorylation in the hypothalamus after ASO IL-10 treatment or after acute recombinant IL-10 infusion. Data are the means ± SEM. # p<0.05 versus chow group; * p<0.05 versus DIO; ¥ p<0.05 versus exercised control animals; n = 8–10 animals per group. Lean animals (white bars), obese animals (black bars), and exercised obese plus recombinant IL-10 (grey bars). SO, sense oligonucleotide; ASO, antisense oligonucleotide.

Figure 6

doi: https://doi.org/10.1371/journal.pbio.1000465.g006