Advertisement

< Back to Article

Circadian and Social Cues Regulate Ion Channel Trafficking

Figure 5

ACTH increases INa magnitude via the cAMP/PKA pathway.

(A) Sodium currents recorded before and after 20 min exposure to saline or ACTH. Sodium currents were pharmacologically isolated by blocking potassium currents with 60 mM TEA. (B) Sodium current increases after 20 min exposure to ACTH. The PKA blocker H89 has no effect alone, but pretreatment with H89 blocked the ACTH-induced increase in Na+ current magnitude (n = 5 per condition, ANOVA F[3, 16] = 9.348, p<0.001). (C) Normalized Na+ I-V curves for cells before and after 20 min saline-control treatment. (D) Normalized Na+ I-V curves for cells before and after 20 min exposure to ACTH. Voltage of peak INa was unchanged in both control and ACTH-treated cells (paired t-test, df = 4, p>0.3 for both saline and ACTH).

Figure 5

doi: https://doi.org/10.1371/journal.pbio.1000203.g005