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Circadian and Social Cues Regulate Ion Channel Trafficking

Figure 2

Circadian and social cues increase EOD amplitude.

(A) Experimental tank used to record calibrated EODs of free-swimming fish. EODs were digitized from nichrome recording electrodes at the ends of the tank only when circuitry detected that the fish was centered within an unglazed ceramic tube, equidistant from the recording electrodes at the ends of the tank. ADC, analog-to-digital-converter. (B) EOD amplitudes of a representative fish recorded approximately every 60 s over 3 d. Signal amplitude shows a clear day-night rhythm increasing to maximum during lights-out and decreasing to a minimum at midday. Inset: superimposed EOD waveforms taken from the same fish at nighttime maximum and daytime minimum. (C) EOD amplitudes were significantly higher at nighttime peak than at daytime minimum (n = 8, t = 3.91, df = 7, p<0.01). Bars show means, and error bars indicate SEM. (D) Adding a second fish into the center compartment for 1 h at midday (arrowheads) caused transient increases in EOD amplitudes of four fish. All voltages are referenced to a point 10 cm from the center of a 5 cm calibration dipole [38].

Figure 2