Negative Regulation of Active Zone Assembly by a Newly Identified SR Protein Kinase
Figure 2
Synaptic Brp deficit in srpk79D mutants.
(A–D) Immunofluorescence images of wild-type and srpk79Datc mutant NMJ reveal a synaptic Brp deficit in srpk79Datc mutants. NMJs are stained with anti-HRP (red) to label the presynaptic membrane and anti-Brp (green). Larvae in wild type and srpk79Datc were stained in the same reaction tube and imaged identically. (E) The total synaptic Brp fluorescence is decreased in srpk79Datc mutants. Each bar graph represents data collected from a total of 30 synapses from nine different larvae. (F) Cumulative frequency plots of synaptic Brp puncta fluorescence intensities are shifted toward smaller values with srpk79D loss of function. Each curve represents data collected from 30 synapses from nine different larvae. n for wild type, srpk79Datc, and srpk79Datc/Df = 6,554, 4,713, and 5,952, respectively. au = arbitrary units; HRP = anti-horseradish peroxidase. (G and H) Disruption of srpk79D affects neither synaptic Brp puncta number (G) nor synaptic bouton number (H). Each bar graph in (G) and (H) represents data collected from a total of 38 synapses taken from 15 different larvae. Scale bar indicates 10 µm. Significance is indicated according to the following: *** = p<0.001 and ns = not significant; Student t-test. Error bars indicate ±SEM.