Dual Regulation by Pairs of Cyclin-Dependent Protein Kinases and Histone Deacetylases Controls G1 Transcription in Budding Yeast
Figure 1
WHI5 overexpression is toxic to strains compromised for Pho85 CDK activity.
Isogenic wt (BY263), pho85Δ (BY391), pcl1Δ (BY628), pcl2Δ (BY451), pcl9Δ (BY694), pcl1Δ pcl9Δ (BY760), and pho80Δ (BY490) strains bearing either GAL1-WHI5 (pBA1973) or empty vector control (pEG-H) were spotted in serial 10-fold dilutions on galactose media and incubated for 72 h at 30°C.