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A Computational Framework for Ultrastructural Mapping of Neural Circuitry

Figure 8

Registering ssTEM Image Tiles with ir-tweak

The entire image represents two windows of the ir-tweak interface. The top window shows two serial sections from a manual film capture with tiles in different orientations (arrows), the left being the fixed and the right the moving or warped image. Successive control points (dots) entered on the fixed image by the user are predictively placed on the moving image based on the model calculated from all previous points, with a thin-plate spline strategy for accommodating local warps. The bottom panel shows the superimposed fixed (blue) and warped (orange) in real-time.

Figure 8