A Computational Framework for Ultrastructural Mapping of Neural Circuitry
(A and B) are mirror images of the transparent overlays of fully overlapping regions of two tiles, both with best alignment centers on a large mitochondrion (white spot). (A) was auto-registered by ir-translate and many membranes appear as double images (arrows) due to nonlinear image distortions between the image pairs.
(B) was registered with ir-grid-refine yielding improved membrane definition, even at very low magnification (resolution is about 5 nm/pixel, which accounts for the blurring). This is a worst-case scenario. With higher resolutions (more pixels) recovery is even more effective. The inset panels are high-pass 3 × 3-pixel filtered patches of the same region, showing severe moiré defects in (A). Scale = 2 μm.