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A Computational Framework for Ultrastructural Mapping of Neural Circuitry

Figure 3

The Workflow for the ssTEM Ultrastructural Circuitry Framework

Parallel serial section grid (ssTEM) and slide (ssLM) libraries are built. The ssLM libraries define either the bounds of the canonical field or are intercalated. Each library is acquired as a set of tiles mosaicked by ir-tools. ssLM and ssTEM mosaics are registered by ir-tweak and ssTEM volumes built with ir-stos applications. CMP classified ssLM imagery is merged with the volume to tag neurons and processes. The volumes are browsed with MosiacBuilder/Viking for process tracking and annotation.

Figure 3

doi: https://doi.org/10.1371/journal.pbio.1000074.g003