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The Bacterial Symbiont Wolbachia Induces Resistance to RNA Viral Infections in Drosophila melanogaster

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Tetracycline Treatment Increases Flies Sensitivity to DCV

(A, B, and C) Fifty 3–6-d-old males, per sample, were injected with DCV, and their survival was followed daily. (A) Flies w1118 iso, VF-0058–3, and VF-0058–3 raised on tetracycline for one generation were injected with 500 TCID50 DCV. (B) Flies w1118 iso and w1118 iso raised on tetracycline were injected with 50 TCID50 DCV. (C) Flies w1118 iso, VF-0058–3, VF-0058–3t, VF-0097–3, and VF-0097–3t were injected with 500 TCID50 DCV. Each assay was repeated once with males and twice with females with similar results.

(D) Fifty 3–6-d-old males, per sample, of VF-0058–3 and VF-0058–3t lines were injected with 50 mM Tris-HCl, pH 7.5, kept at 18°C and their survival was followed daily.

(E) Extracts of VF-0058–3 and VF-0058–3t flies 3 and 6 d after injection with 500 TCID50 DCV or not injected were probed in a Western blot with anti-DCV. Anti-tubulin was used as a loading control.

(F) Titration, in cell culture, of DCV levels per fly of VF-0058–3 and VF-0058–3t flies 3 and 6 d after injection with 500 TCID50 DCV. Squares are replicates (four per sample), lines are geometric means of replicates. Virus titres in VF-0058–3 and VF-0058–3t are significantly different on both days post-infection (Mann-Whitney test, p = 0.0287 for both comparisons).

Figure 1

doi: https://doi.org/10.1371/journal.pbio.1000002.g001