Microsporidia Are Natural Intracellular Parasites of the Nematode Caenorhabditis elegans
Figure 7
N. parisii Infection in Wild-Type and pmk-1 Mutant Animals
(A and C) Comparison of infection symptoms in wild-type and pmk-1 mutants infected with N. parisii at the L1 stage and assayed at 43 h postinoculation (A) or infected at the L4 stage and then assayed 47 h postinoculation (C), with at least 40 animals per plate. The average of three plates is shown, error bars are standard deviation. There was not a significant difference between wild-type and pmk-1 at the L1 stage (p = 0.75 for meronts, p = 0.39 for spores) or the L4 stage (p = 0.10 for meronts, p = 0.53 for spores).
(B and D) Comparison of survival between wild-type and pmk-1(km25) mutants inoculated with N. parisii at L1 stage (B) or L4 stage (D). Assays were performed starting with at least 40 animals per plate, three plates per experiment. In (D), “un” refers to uninfected. There was not a significant difference between wild-type and pmk-1 at the L1 stage (p = 0.0529) or the L4 stage (p = 0.65).
(E) Comparison of infection symptoms in wild-type, daf-2(e1368), and daf-2(e1368);daf-16(mgDf47) mutants infected with N. parisii at the L4 stage and assayed at 45 h postinoculation. The average of three plates is shown, error bars are standard deviation. There was not a significant difference between wild-type and daf-2 (p = 0.69 for meronts, p = 0.44 for spores), between wild-type and daf-2;daf-16 (p = 0.15 for meronts, p = 0.22 for spores) or between daf-2 and daf-2;daf-16 (p = 0.19 for meronts, p = 0.17 for spores).
(F) Comparison of survival between wild-type, daf-2(e1368), and daf-2(e1368);daf-16(mgDf47) mutants infected with N. parisii at the L4 stage. Assays were performed starting with at least 40 animals per plate, three plates per experiment. There was a significant difference between survival of wild-type and daf-2 (p < 0.0001) and between daf-2 and daf-2;daf-16 (p < 0.0001), but not between wild-type and daf-2;daf-16 (p = 0.40). All data shown in the figure are representative of at least three independent experiments.