Biogenesis of the Trypanosome Endo-Exocytotic Organelle Is Cytoskeleton Mediated
Figure 4
RNAi Knockdown of BILBO1 Induces Loss of Basal Body–Mediated Golgi Segregation and Causes Defects of Important Cytoskeletal Structures
(A–C) A nontransformed 2K2N cell probed with anti-GRASP (green) and DAPI (blue), illustrating two major GRASP signals (arrowheads) located between the segregated kinetoplasts and nuclei.
(D–F) A BILBO1 RNAi-induced (36 h) 2K2N cell probed with anti-GRASP. The two GRASP signals are observed near the nuclei. Despite a limited degree of Golgi segregation, no GRASP signal is observed near the new kinetoplast. The kinetoplast and the new flagellum (asterisk) are located in the extreme posterior end of the cell.
(G–I) BILBO1 and the FPC are important for cytoskeleton organisation. Immunofluorescence micrograph of a PF cytoskeleton probed with L3B2 (anti-FAZ) antibody after BILBO1 RNAi knockdown (36 h). The flagellum-to-cell body attachment is lost, and the new flagellum is located at the posterior region of the cell. No new FAZ is formed, whereas the old FAZ remains associated with the old flagellum. The kinetoplast (asterisk) is located in the extreme posterior of the cell.
Scale bar indicates 5 μm.