Creatine Kinase–Mediated ATP Supply Fuels Actin-Based Events in Phagocytosis
Figure 8
Coupling between CK-B Activity, Actin Polymerization State, and Phagocytosis
Adhesion assays were performed with RAW 264.7 cells, pretreated with cytochalasin D (50 or 100 nM) and COZ (A) or IgG-opsonized zymosan (B). The total number of particles (adherent and internalized) was determined and normalized to nontreated cells (averages of 3–4 experiments ± SD).
(C) Raw 264.7 cells were treated with 5 mM cCr prior to F-actin quantification using fluorescently labeled phalloidin (averages of four experiments ± SD). *p < 0.05; **p < 0.01; ***p < 0.001.