Creatine Kinase–Mediated ATP Supply Fuels Actin-Based Events in Phagocytosis
Figure 6
CK-B and CK-B(C283S) Influence Phagocytosis
(A) Western blot analysis of RAW 264.7 cells stably expressing CK-B or CK-B(C283S). Two individual retrovirally transduced populations were maintained (CK-B#1 and CK-B#2, and CK-B(C283S)#1 and CK-B(C283S)#2). RAW 264.7 cells expressing EYFP and noninfected cells were used as control.
(B) CK enzymatic activity of depicted cell lysates.
Fluorescent particle uptake capacity quantified by FACS in cell lines incubated with zymosan (C), COZ (D), or IgG zymosan (E).
Bars represent the average of three to four experiments (±SD). *p < 0.05; **p < 0.005; ***p < 0.0005.