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Selective Inflammatory Pain Insensitivity in the African Naked Mole-Rat (Heterocephalus glaber)

Figure 5

Patch Clamp Recordings of Spinal Cells in Superficial and Deep Lamina of the Dorsal Horn with Bath Application of Capsaicin (10 μM)

(A and B) Locations of cells in mouse (A) and naked mole-rat (B) spinal slices. White dots indicate cells that responded to capsaicin; black dots indicate cells that did not respond to capsaicin. Scale bar = 100 μm.

(C) Mean number of mEPSCs recorded from all superficial mouse cells that responded to capsaicin. The histogram shows 100 s before and 100 s after bath application of capsaicin. The inset trace shows a typical recording from one mouse cell. Scale bars, 10 s, 10 pA.

(D) Mean number of mEPSCs from four naked mole-rat cells that were located in superficial dorsal horn and responded to capsaicin.

(E) Mean number of mEPSCs from six naked mole-rat cells that were located in deep dorsal horn and responded to capsaicin. Miniature EPSCs were recorded in the presence of strychnine (1 μM), picrotoxin (100 μM), and APV (100 μM) to block glycinergic and GABAergic input as well as NMDA receptor-mediated currents. TTX (1 μM) was used to block action-potential dependent neurotransmitter release. Bin size for (C–E), 5 s.

Figure 5

doi: https://doi.org/10.1371/journal.pbio.0060013.g005