PER-TIM Interactions with the Photoreceptor Cryptochrome Mediate Circadian Temperature Responses in Drosophila
Figure 1
Heat Pulse–Mediated Phase Delays of Wild-Type Flies Require 37 °C
Phase response curves for CS flies after heat (circle) versus light pulse (square). Flies were entrained for 3 d in 12 h:12 h LD cycles and pulsed for 30 min of light and 30 °C, 34 °C, and 37 °C heat pulse (HP) during the last night of the LD entrainment cycle, after which the flies were released in constant darkness for 5 d. Phase changes were calculated by comparing behavioral offsets of light or HP treated flies 3 d after the pulse to the behavior of the control group of the same genotype that did not receive a pulse. The calculations were made by MATLAB software using previously described methods [46]. Phase delays and phase advances are plotted (± SEM) as negative and positive values respectively. In all cases, the experiments were repeated at least twice with similar results. Data were pooled from the following number of flies (each pair of values referring to wild-type light-pulsed and wild-type 37 °C heat-pulsed): control: 32, 32; pulse at ZT12: 32, 22; pulse at ZT15: 32, 26; pulse at ZT18: 23, 26; pulse at ZT21: 24, 19; and pulse at ZT24: 29, 27.