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Linking Splicing to Pol II Transcription Stabilizes Pre-mRNAs and Influences Splicing Patterns

Figure 5

The Link between Pol II Transcription and Pre-mRNA Splicing Increases the Affinity of Splicing Factors to the Nascent Pre-mRNA

A mathematical model is used to describe the basic reactions involved in the coupled transcription/splicing reaction. The splicing profiles obtained from RNAs generated by Pol II (A) or by T7 polymerase (B) were fit to the mathematical prediction by testing various affinities (Km) between the splicing machinery and the pre-mRNA. (A and B) represent the best fit for either reaction. Squares represent pre-mRNA and triangles represent spliced mRNA. The solid line represents the mathematical prediction for the accumulation of pre-mRNA based on the Km value indicated below each graph. The dotted line represents the mathematical prediction for the accumulation of spliced mRNA based on the Km value indicated below each graph.

Figure 5

doi: https://doi.org/10.1371/journal.pbio.0040147.g005