Atypical Membrane Topology and Heteromeric Function of Drosophila Odorant Receptors In Vivo
(A) Immunostaining for GFP:OR43a (α-GFP, green) and OR83b (red) when misexpressed singly or in combination in Gr21a neurons. These animals are homozygous mutant for Or83b, which allows visualization of protein distributions specifically in Gr21a neurons. The arrowhead marks very weak localization of OR83b to cilia when expressed alone. Genotypes: Top row: Gr21a-Gal4/UAS-GFP:Or43a;Or83b1/Or83b2. Middle row: Gr21a-Gal4/+;UAS-Or83b,Or83b1/Or83b2. Bottom row: Gr21a-Gal4/UAS-GFP:Or43a;UAS-Or83b,Or83b1/Or83b2.
(B) Immunostaining for GFP:OR43a (α-GFP, green) and OR83b (red) when misexpressed singly or in combination in second antennal segment mechanosensory neurons using the ciliated cell-specific oseg2-Gal4 driver. OR83b shows extremely weak localization to cilia that is not visible under these imaging conditions. Merged images are overlaid on a bright-field image to visualize tissue morphology. Genotypes: Top row: oseg2-Gal4/UAS-GFP:Or43a. Middle row: oseg2-Gal4/+;UAS-Or83b/+. Bottom row: oseg2-Gal4/UAS-GFP:Or43a;UAS-Or83b/+.