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Atypical Membrane Topology and Heteromeric Function of Drosophila Odorant Receptors In Vivo

Figure 3

Spatial Requirements for OR83b in OR Localization

(A) Immunostaining of GFP:OR43a (α-GFP, green) expressed in all Or83b neurons and OR83b (red) in antennal sections of control heterozygous (Or83b-Gal4/UAS-GFP:Or43a;Or83b1/+, left panels) and homozygous (Or83b-Gal4/UAS-GFP:Or43a;Or83b1/Or83b2, right panel) Or83b null-mutant animals.

(B) Immunostaining for GFP:OR47b (α-GFP, green) expressed in Or47b neurons and OR83b (red) in antennal sections of control heterozygous (Or47b-Gal4/UAS-GFP:Or47b;Or83b1/+, left panels) and homozygous (Or47b-Gal4/UAS-GFP:Or47b;Or83b1/Or83b2, right panel) Or83b null-mutant animals. Arrowheads illustrate low level of OR83b expression in Or47b neurons.

(C) Immunostaining for GFP:GR21a (α-GFP, green) expressed in Gr21a neurons and OR83b (red) in antennal sections of control heterozygous (Gr21a-Gal4/UAS-GFP:Gr21a;Or83b1/+, left panel) and homozygous (Gr21a-Gal4/UAS-GFP:Gr21a;Or83b1/Or83b2, right panel) Or83b null-mutant animals. The OR83b signal in this sensillum reflects expression in the non-Gr21a-expressing neurons ab1A, ab1B, and ab1D, whose olfactory responses are Or83b-dependent [22,32].

(D) Immunostaining for GFP:GR21a (α-GFP, green) expressed in all Or83b neurons and OR83b (red) in antennal sections of wild-type animals (Or83b-Gal4/+;UAS-GFP:Gr21a/+).

Figure 3

doi: https://doi.org/10.1371/journal.pbio.0040020.g003