DNA Barcoding: Error Rates Based on Comprehensive Sampling
(A) Relative distributions of intraspecific variability (coalescent depth—red) and interspecific divergence between ESUs (yellow), demonstrating significant overlap and the lack of a barcoding gap. Note that the x-axis scale shifts to progressively greater increments above 0.02.
(B) Cumulative error based on false positives plus false negatives for each threshold value. The optimum threshold value is 0.013 (2.6%), where error is minimized at 17%.