Rab7 Associates with Early Endosomes to Mediate Sorting and Transport of Semliki Forest Virus to Late Endosomes
Figure 8
Schematic Representation of the SFV Transport Pathway from the Plasma Membrane to Lysosomes
After uncoating of clathrin, the primary endocytic vesicles deliver the virus to Rab5-positive early endosomes, which contain Rab5 and Arf1 domains but do not contain Rab7. Rab7 is then recruited to these endosomes, and it forms additional domains (blue) that exclude the other early endosome-associated small GTPases. The virus moves into the Rab7 domain, and when this domain leaves the endosome as a Rab7-containing transport vesicle, the virus also leaves the Rab5-positive organelle. The Rab7-containing carrier moves along microtubules and fuses with other carriers or late endosomes. Also shown is an alternative route from the early to the late endosome, involving Arf1 and the ECV, which is not used by SFV. CCP, clathrin-coated pit; CCV, clathrin-coated vesicle; EE, early endosome; MT, microtubule; RE, recycling endosome; LE, late endosome; Lys, lysosome.