Cobalamin-Independent Methionine Synthase (MetE): A Face-to-Face Double Barrel That Evolved by Gene Duplication
Figure 6
Superposition of the Hcy Binary Complex (Yellow) and Substrate-Free (Gray) Enzymes Showing Local Changes in the DMV Region
Met468 moves toward the Hcy substrate, rearranging the start of helix α2AH, and a new hydrogen bond is formed between the carbonyls of Met468 and Thr531. This position of Met468 stabilizes a rotamer of Trp539 that favors folate binding.