A Spontaneous, Recurrent Mutation in Divalent Metal Transporter-1 Exposes a Calcium Entry Pathway
Figure 5
DMT1-Like and G185R-Like Currents in Enterocytes Isolated from Wild-Type and mk/mk Mice, Respectively
(A) Enterocyte currents isolated from an iron-deficient wild-type mouse (−Fe). Reducing bath pH (140 mM NaCl) induced a slowly desensitizing inward current that was further enhanced by addition of Mn2+.
(B) Both proton and H+/Mn2+currents were inwardly rectifying.
(C and D) An mk enterocyte expressed a large constitutive inward current in control bath solution. Reducing the bath pH (140 mM NaCl) first inhibited and then activated another inward current insensitive to the holding potential. This slowly-desensitizing current displayed a less steeply rectifying I-V as shown in (D).