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Aneuploidy always damages animal cells

Posted by drasnick on 30 Jul 2009 at 14:09 GMT

Single-cell organisms, even if eukaryotic, are not a suitable experimental model to study the effects of aneuploidy on the growth and other properties of animal cells.

While it is certainly true that aneuploidy alters the growth characteristics of cells, aneuploidy always produces animal cells that are damaged and less viable compared to euploid cells (Atkin, N. B. and Baker, M. C. (1990) Cytogenetics and Cell Genetics 53, 58-60, Sandler, L. and Hecht, F. (1973) American Journal of Human Genetics 25, 332-339, Lindsley, D. L., Sandler, L. and et al. (1972) Genetics 71, 157-184, German, J. (1974) In Chromosomes and Cancer. vol. 1 (German, J., ed.), pp. 601-617, John Wiley & Sons, New York, Rasnick, D. and Duesberg, P. H. (1999) Biochemical Journal 340, 621-630).

Aneuploid cancer cells are always damaged and less viable than their euploid counterparts. The growth advantage possessed by some aneuploid animal cells is not superior growth, but simply their ability to survive in vivo and continue dividing at diminished rates.

No competing interests declared.

RE: Aneuploidy always damages animal cells

biktye replied to drasnick on 30 Jul 2009 at 17:23 GMT

Obviously, I disagree with the first statement.

The rest of the comments about animal cells studies support our report in this paper that aneuploidy retards growth in our yeast model.

No competing interests declared.

RE: Aneuploidy always damages animal cells

Vinny replied to drasnick on 10 Aug 2009 at 20:33 GMT

I strongly agree with drasnick. Plus the variation of colony size on yeast plates appears pretty normal to me even without any mutations, and I always avoided picking up those small sick colonies for further study. I wonder how the initial normal sized MCM4(Chaos3/Chaos3) colonies would behave in the following tests.

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RE: RE: Aneuploidy always damages animal cells

Karma replied to Vinny on 21 Apr 2010 at 02:43 GMT

There is variation of the colony size and most strains we checked have normal distribution. The chaso3/chaos3 strain draws our attention because they have way more minute colonies which leads to a bimodal distribution. I personally believe the minute colony represents a hypermutable stage which have been hypothesized for tumor transformation.

As you said, normally people will not pick those colonies for further study. That is way I have to create a methodology to study and describe the characteristic of those minute colonies.

For the initial normal sized MCM4(chaso3/Chaso3), we have shown that they are slow growth and show hyperrecombination. The initial normal sized colonies after 30 days of passage, around 25% of them gain the trait of accelerated proliferation and aneuploidy.

No competing interests declared.

RE: Aneuploidy always damages animal cells

Karma replied to drasnick on 21 Apr 2010 at 02:31 GMT

I have to say the first statement is just drasnick's own opinion, and I do not see why we cannot learn about the effect of aneuploidy in yeast.

There is no evidence that aneuploidy is harmful in tumor cells with altered karyotype, since there is no way to remove the aneuploidy. I think the yeast model provides us a unique chance to remove the aneuploidy from those fast proliferating cells.

Most cancer cells are aneuploid, and they proliferate faster than the euploid normal cells. That is the reason some people believe that identifying the type of chromosome changes in different tumor will help us to understand cancer.

No competing interests declared.