Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

Chen Wang; Jiayan Sang; Jiawei Wang; Mingyan Su; Jennifer S. Downey; Qinggan Wu; Shida Wang; Yongfei Cai; Xiaozheng Xu; Jun Wu; Dilani B. Senadheera; Dennis G. Cvitkovitch; Lin Chen; Steven D. Goodman; Aidong Han

doi:10.1371/journal.pbio.1001493

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Peer-reviewed

Research Article

Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

Chen Wang ,

Contributed equally to this work with: Chen Wang, Jiayan Sang

Affiliations State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China, Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States of America
⨯
Jiayan Sang ,

Contributed equally to this work with: Chen Wang, Jiayan Sang

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Jiawei Wang,

Affiliation Department of Biology and Technology, Tsinghua University, Beijing, China
⨯
Mingyan Su,

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Jennifer S. Downey,

Affiliation Division of Biomedical Science, Herman Ostrow School of Dentistry of University of Southern California, Los Angeles, California, United States of America
⨯
Qinggan Wu,

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Shida Wang,

Affiliation Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
⨯
Yongfei Cai,

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Xiaozheng Xu,

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Jun Wu,

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯
Dilani B. Senadheera,

Affiliation Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
⨯
Dennis G. Cvitkovitch,

Affiliation Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
⨯
Lin Chen,

Affiliation Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States of America
⨯
Steven D. Goodman,

Affiliation Division of Biomedical Science, Herman Ostrow School of Dentistry of University of Southern California, Los Angeles, California, United States of America
⨯
Aidong Han

* E-mail: ahan@xmu.edu.cn

Affiliation State Key Laboratory for Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan, Xiamen, China
⨯

Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

Chen Wang,
Jiayan Sang,
Jiawei Wang,
Mingyan Su,
Jennifer S. Downey,
Qinggan Wu,
Shida Wang,
Yongfei Cai,
Xiaozheng Xu,
Jun Wu

Published: February 26, 2013
https://doi.org/10.1371/journal.pbio.1001493

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PLOS Biologue blog post

Posted by rolir on 28 Feb 2013 at 18:01 GMT

See the blog post on PLOS Biologue: http://blogs.plos.org/bio...

No competing interests declared.

RE: PLOS Biologue blog post

ahan replied to rolir on 08 Mar 2013 at 01:56 GMT

Dr. Roberts has created a great picture by putting together ours with the work from Dr. Crane and his colleague. I strongly suggest he write another comprehensive comment/review about this idea for a formal publication. The two things I like to point out here. One is that VicK HAMP has unique packing (Figure 2), which may be more stable than other homologues. How the HAMP changes its conformation is still puzzling to us. The second is that VicK PAS domain is not found to bind by any ligands. This is a major drawback of VicK used in studying molecular mechanisms of signal transduction. Therefore, we have not been able to analyze any upstream conformational changes in context with downstream DHp domain bending and CA positioning in this paper. We are currently performing molecular dynamics study, hoping to find some insights of conformational transitions.

No competing interests declared.

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Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

Mechanistic Insights Revealed by the Crystal Structure of a Histidine Kinase with Signal Transducer and Sensor Domains

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PLOS Biologue blog post

Posted by rolir on 28 Feb 2013 at 18:01 GMT

RE: PLOS Biologue blog post

ahan replied to rolir on 08 Mar 2013 at 01:56 GMT