Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

Anita B. Hjelmeland; Qiulian Wu; Sarah Wickman; Christine Eyler; John Heddleston; Qing Shi; Justin D. Lathia; Jennifer MacSwords; Jeongwu Lee; Roger E. McLendon; Jeremy N. Rich

doi:10.1371/journal.pbio.1000319

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Open Access

Peer-reviewed

Research Article

Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

Anita B. Hjelmeland ,

* E-mail: hjelmea@ccf.org (ABH); richj@ccf.org (JNR)

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Qiulian Wu,

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Sarah Wickman,

Affiliation Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Christine Eyler,

Affiliations Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
John Heddleston,

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Qing Shi,

Affiliation Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Justin D. Lathia,

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Jennifer MacSwords,

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Jeongwu Lee,

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯
Roger E. McLendon,

Affiliation Department of Pathology, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Jeremy N. Rich

* E-mail: hjelmea@ccf.org (ABH); richj@ccf.org (JNR)

Affiliation Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
⨯

Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

Anita B. Hjelmeland,
Qiulian Wu,
Sarah Wickman,
Christine Eyler,
John Heddleston,
Qing Shi,
Justin D. Lathia,
Jennifer MacSwords,
Jeongwu Lee,
Roger E. McLendon

Published: February 23, 2010
https://doi.org/10.1371/journal.pbio.1000319

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Publisher's Note: Error in the Immunofluorescence Staining section

Posted by PLOSBiology on 04 May 2010 at 00:53 GMT

Cells, neurospheres, or tumor sections were fixed with 4% paraformaldehyde, washed with Tris-buffered saline, and incubated with polyclonal mouse anti-A20 (Santa Cruz Biotechnology) and goat anti-Sox2 (Santa Cruz Biotechnology) where indicated.
http://plosbiology.org/article/info:doi/10.1371/journal.pbio.1000319#article1.body1.sec4.sec4.p1

There was an error in the first sentence of the Immunofluorescence Staining section. The correct sentence is: Cells, neurospheres, or tumor sections were fixed with 4% paraformaldehyde, washed with Tris-buffered saline, and incubated with mouse anti-A20 (Santa Cruz Biotechnology) and rabbit anti-Sox2 (Santa Cruz Biotechnology) where indicated.

No competing interests declared.

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Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth

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Publisher's Note: Error in the Immunofluorescence Staining section

Posted by PLOSBiology on 04 May 2010 at 00:53 GMT