About the Authors

Keith I Pardee

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada

Xiaohui Xu

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada , Midwest Center for Structural Genomics, University of Toronto, Toronto, Canada

Jeff Reinking

Current address: Department of Structural and Chemical Biology, Ichah Medical Institute, Mount Sinai School of Medicine, New York, New York, United States of America

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada , Department of Biology, State University of New York at New Paltz, New Paltz, New York, United States of America

Anja Schuetz

Current address: Protein Sample Production Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Canada

Aiping Dong

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Canada

Suya Liu

Affiliation Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

Rongguang Zhang

Affiliation Midwest Center for Structural Genomics, Argonne National Lab, Argonne, Illinois, United States of America

Jens Tiefenbach

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada

Gilles Lajoie

Affiliation Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

Alexander N Plotnikov

Current address: Protein Sample Production Facility, Max Delbrück Center for Molecular Medicine, Berlin, Germany

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Canada

Alexey Botchkarev

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Canada

Henry M Krause

To whom correspondence should be addressed. E-mail: aled.edwards@utoronto.ca (AE); h.krause@utoronto.ca (HMK)

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada

Aled Edwards

To whom correspondence should be addressed. E-mail: aled.edwards@utoronto.ca (AE); h.krause@utoronto.ca (HMK)

Affiliations Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada , Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada , Midwest Center for Structural Genomics, University of Toronto, Toronto, Canada , Structural Genomics Consortium, University of Toronto, Toronto, Canada

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

KIP, JR, AS, JT, ANP, HMK, and AE conceived and designed the experiments. KIP, XX, JR, AS, AD, SL, and RZ performed the experiments. KIP, JR, AS, AD, SL, JT, ANP, AB, HMK, and AE analyzed the data. GL contributed reagents/materials/analysis tools. KIP, HMK and AE wrote the paper.