About the Authors

Julian R Hughes

Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

Current address: Sareum Ltd., Cambridge, United Kingdom

Affiliation Department of Zoology, University of Oxford, Oxford, United Kingdom

Ana M Meireles

Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

Affiliations Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom , Programa Doutoral em Biologia Experimental e Biomedicina, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal

Katherine H Fisher

Contributed equally to this work with: Julian R Hughes, Ana M Meireles, Katherine H Fisher

Affiliations Department of Zoology, University of Oxford, Oxford, United Kingdom , Life Sciences Interface/Doctoral Training Centre, University of Oxford, Oxford, United Kingdom , Department of Biochemistry, University of Oxford, Oxford, United Kingdom

Angel Garcia

Current address: Universidade de Santiago de Compostela, Santiago de Compostela, Spain

Affiliation Department of Statistics, University of Oxford, Oxford, United Kingdom

Philip R Antrobus

Affiliation Department of Statistics, University of Oxford, Oxford, United Kingdom

Alan Wainman

Affiliation Department of Zoology, University of Oxford, Oxford, United Kingdom

Nicole Zitzmann

Affiliation Department of Statistics, University of Oxford, Oxford, United Kingdom

Charlotte Deane

Affiliation Department of Biochemistry, University of Oxford, Oxford, United Kingdom

Hiroyuki Ohkura

Affiliation Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom

James G Wakefield

To whom correspondence should be addressed. E-mail: james.wakefield@zoo.ox.ac.uk

Affiliations Department of Zoology, University of Oxford, Oxford, United Kingdom , Life Sciences Interface/Doctoral Training Centre, University of Oxford, Oxford, United Kingdom

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

The MT cosedimentation assays were undertaken by JRH and JGW. Proteomic analyses were carried out by AG and PRA, and supervised by NZ. All RNAi experiments were undertaken by AMM and supervised by HO. Bioinformatics analyses were undertaken by KHF and supervised by CD. Biochemical analyses of complexes were carried out by JRH and KHF. Immunolocalisation of complexes and the localisation of GFP-SkAP were undertaken by AW. The overall experimental rationale was designed by JRH and JGW.