About the Authors

Abdellah Allali-Hassani

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Patricia W Pan

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada

Ludmila Dombrovski

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Rafael Najmanovich

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , European Bioinformatics Institute, Cambridge, United Kingdom

Wolfram Tempel

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Aiping Dong

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Peter Loppnau

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Fernando Martin

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Janet Thonton

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , European Bioinformatics Institute, Cambridge, United Kingdom

Aled M Edwards

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada , Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada

Alexey Bochkarev

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada

Alexander N Plotnikov

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , Department of Physiology, University of Toronto, Toronto, Ontario, Canada

Masoud Vedadi

Affiliation Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada

Cheryl H Arrowsmith

To whom correspondence should be addressed. E-mail: carrow@uhnres.utoronto.ca

Affiliations Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada , Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada , Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

AAH performed all binding assays, developed the SULT activity assay, and performed some of the activity assays. PWP performed many of the activity assays, purified the proteins for activity and binding assays, and crystallized SULT1C2 with PAP and PCP. LD purified and crystallized all other proteins whose structures are reported here. RN performed structure, binding, and activity profile analysis. WT solved the structure of SULT1C2 with PAP and PCP, and performed structural analysis. AD helped with X-ray data collection and crystallization, and solved the structures of SULTs 1B1, 1C1, 1C2, and 4A1. PL generated all the expression clones. FM performed computational analysis of SULT4A1 and SULT1C2 with PAP and PCP, and PCBs. JT contributed to data analysis and the manuscript. AME contributed to overall project design and the manuscript. AB led the crystallographic part of the project and contributed to data analysis and the manuscript. ANP led the structural genomics part of the project and contributed to structure analysis and the manuscript. MV led the screening part of the project and contributed to data analysis and the manuscript. CHA contributed to overall project design and data analysis, and led the preparation of the manuscript. AAH, PWP, LD, RN, ANP, and MV contributed equally to this paper.