About the Authors
- Raffi Tonikian
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Contributed equally to this work with: Raffi Tonikian, Yingnan Zhang
Affiliations Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada , Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
- Yingnan Zhang
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Contributed equally to this work with: Raffi Tonikian, Yingnan Zhang
Affiliation Department of Protein Engineering, Genentech, South San Francisco, California, United States of America
- Stephen L Sazinsky
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Affiliation Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
- Bridget Currell
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Affiliation Department of Molecular Biology, Genentech, South San Francisco, California, United States of America
- Jung-Hua Yeh
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Affiliation Department of Immunology, Genentech South San Francisco, California, United States of America
- Boris Reva
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Affiliation Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
- Heike A Held
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Affiliation Department of Protein Engineering, Genentech, South San Francisco, California, United States of America
- Brent A Appleton
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Affiliation Department of Protein Engineering, Genentech, South San Francisco, California, United States of America
- Marie Evangelista
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Affiliation Department of Molecular Biology, Genentech, South San Francisco, California, United States of America
- Yan Wu
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Affiliation Department of Antibody Engineering, Genentech, South San Francisco, California, United States of America
- Xiaofeng Xin
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Affiliations Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada , Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
- Andrew C Chan
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Affiliation Department of Immunology, Genentech South San Francisco, California, United States of America
- Somasekar Seshagiri
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Affiliation Department of Molecular Biology, Genentech, South San Francisco, California, United States of America
- Laurence A Lasky
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Affiliation Department of Protein Engineering, Genentech, South San Francisco, California, United States of America
- Chris Sander
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Affiliation Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
- Charles Boone
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*To whom correspondence should be addressed. E-mail: sachdev.sidhu@utoronto.ca (SSS); gary.bader@utoronto.ca (GDB); charlie.boone@utoronto.ca (CB)
Affiliations Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada , Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
- Gary D Bader
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*To whom correspondence should be addressed. E-mail: sachdev.sidhu@utoronto.ca (SSS); gary.bader@utoronto.ca (GDB); charlie.boone@utoronto.ca (CB)
¤ Current address: Terrence Donnelly Center for Cellular and Biomolecular Research and Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
Affiliations Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada , Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada , Computational Biology Center, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
- Sachdev S Sidhu
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*To whom correspondence should be addressed. E-mail: sachdev.sidhu@utoronto.ca (SSS); gary.bader@utoronto.ca (GDB); charlie.boone@utoronto.ca (CB)
¤ Current address: Terrence Donnelly Center for Cellular and Biomolecular Research and Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
Affiliation Department of Protein Engineering, Genentech, South San Francisco, California, United States of America
Competing Interests
YZ, BC, J-HY, BAA, ME, YW, ACC, SS, LAL, and SSS are employed by Genentech.
Author Contributions
LAL, CS, CB, GDB, and SSS conceived and designed the experiments. RT and YZ performed the phage display experiments. SLS performed the Erbin point mutant experiments. J-HY and ACC conceived and performed the cell-based assays. BC and SS performed the DNA sequencing. J-HY, HAH, ME, YW, and XX provided materials and reagents. BR and GDB performed the computational analysis. BAA and SSS performed the structural analysis. RT, GDB, and SSS produced the figures and wrote the manuscript. LAL, CS, and CB revised the manuscript.