About the Authors
- Martin Wu
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Ling V Sun
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Affiliation Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut, United States of America
- Jessica Vamathevan
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Markus Riegler
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Affiliation Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, St Lucia, Queensland, Australia
- Robert Deboy
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Jeremy C Brownlie
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Affiliation Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, St Lucia, Queensland, Australia
- Elizabeth A McGraw
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Affiliation Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, St Lucia, Queensland, Australia
- William Martin
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Affiliation Institut für Botanik III, Heinrich-Heine Universität, Düsseldorf, Germany
- Christian Esser
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Affiliation Institut für Botanik III, Heinrich-Heine Universität, Düsseldorf, Germany
- Nahal Ahmadinejad
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Affiliation Institut für Botanik III, Heinrich-Heine Universität, Düsseldorf, Germany
- Christian Wiegand
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Affiliation Institut für Botanik III, Heinrich-Heine Universität, Düsseldorf, Germany
- Ramana Madupu
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Maureen J Beanan
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Lauren M Brinkac
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Sean C Daugherty
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- A. Scott Durkin
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- James F Kolonay
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- William C Nelson
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Yasmin Mohamoud
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Perris Lee
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Kristi Berry
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- M. Brook Young
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Teresa Utterback
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Janice Weidman
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- William C Nierman
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Ian T Paulsen
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Karen E Nelson
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Hervé Tettelin
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Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
- Scott L O'Neill
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Affiliations Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut, United States of America, Department of Zoology and Entomology, School of Life Sciences, The University of Queensland, St Lucia, Queensland, Australia
- Jonathan A Eisen
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*To whom correspondence should be addressed. E-mail: jeisen@tigr.org
Affiliation The Institute for Genomic Research, Rockville, Maryland, United States of America
Competing Interests
The authors have declared that no conflicts of interest exist.
Author Contributions
M. Wu contributed ideas and analysis in all aspects of the work. L. Sun performed purification of wMel DNA for initial libraries and closure. J. Vamathevan was the closure team leader, performed sequence assembly and analysis, and screened contigs against the Drosophila genome. M. Riegler performed validation of assembly against the physical map and confirmation of rearrangements by long PCR and analysis of repeat regions. R. Deboy was the annotation leader and managed the annotation, ORF management, and frameshifts. J. C. Brownlie performed analysis of Type IV secretion systems. E. A. McGraw performed validation of assembly against physical map and confirmation of rearrangements by long PCR and analysis of wsp paralogs. W. Martin, C. Esser, N. Ahmadinejad, and C. Wiegand performed the mitochondrial evolution analysis. R. Madupu, M. J. Beanan, L. M. Brinkac, S. C. Daugherty, A. S. Durkin, J. F. Kolonay, and W. C. Nelson performed genome annotation. Y. Mohamoud, P. Lee, and K. Berry performed the closure experiments (closed sequencing gaps, multiplex PCR, resolution of small repeats, coverage reactions, contig editing, resolution of large repeats by transposon and primer walking). M. B. Young was the shotgun sequencing leader. T. Utterback and J. Weidman performed shotgun sequencing and frameshift checking; Utterback also worked on the assembly. W. C. Nierman handled the library construction. I. T. Paulsen performed transporter analysis. K. E. Nelson performed metabolism analysis. H. Tettelin analyzed genome properties, repeats, and membrane proteins. S. L. O'Neill and J. A. Eisen supplied ideas, coordination, and analysis; Eisen is the corresponding author.